Resposta de neutrófilos e monócitos do sangue periférico ao LPS de bactérias lisas e rugosas / Neutrophils and monocytes response to LPS from smooth and rough bacteria

AUTOR(ES)

Natalia Estevam Gomes

FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

27/02/2008

RESUMO

Objective: To compare the biological action of R form and S form LPS in human peripheral blood neutrophils and monocytes. Methods: Cell surface receptors modulation, ROS and NO production were evaluated on monocytes and neutrophils in whole blood, and NF-êB activation was evaluated in isolated neutrophils by flow cytometry after incubation with R and S-LPS. Results: A small enhancement of TLR4 expression was observed on monocytes with 6 h stimulation whereas no modulation of TLR2 was seen on either cell. Modulation of CD14 on monocytes was biphasic, with initial increment in the first hour followed by decreased expression at 6 h. Both LPS increased CD66b expression on neutrophils. Expression of CD11b was rapidly up-regulated on monocytes and neutrophils by both LPS, while down regulation of CXCR2 expression on neutrophils was observed after a few minutes, lasting for 6 h. S-LPS effect on neutrophils expression of CD11b, CD66b and CXCR2 receptors was a little earlier than the R-LPS effect. Both LPS induced low production of ROS and NO compared to other stimuli, as well as NF-êB translocation. Conclusions: A complex LPS-induced cell surface receptors modulation was seen on monocytes and neutrophils in human blood, with up and down regulation depending on the receptors evaluated. R for and S form LPS have similarly activated human neutrophils, despite the low expression of CD14 in those cells. While the presence of LBP and of sCD14 could provide the requirements for S-form LPS induction of neutrophils activity in whole blood, other receptors are also likely to be involved in LPS cell signaling in neutrophils.

ASSUNTO(S)

antígenos cd14 ativação de neutrófilo receptores da superfície celular proteínas de membrana monócitos doencas infecciosas e parasitarias

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