Response of Salmonella choleraesuis LT2 Spheroplasts and Permeabilized Cells to the Bacteriocin AS-48
AUTOR(ES)
Abriouel, H.
FONTE
American Society for Microbiology
RESUMO
The bacteriocin AS-48 was not active against intact cells of Salmonella choleraesuis LT2 at neutral pH, but it was very effective on spheroplasts, suggesting that the outer membrane (OM) acts as a protective barrier. Cells sublethally injured by heat or treated with OM-permeabilizing agents (i.e., EDTA and Tris) became sensitive to AS-48. The combination of two or more treatments decreased the amount of AS-48 required for cell killing. The activity of AS-48 against heat-injured cells did not change significantly in the pH range of 4.0 to 8.0. AS-48 showed bactericidal activity against intact cells of Salmonella at pH 4.0. The potency of AS-48 increased greatly when the bacteriocin was dissolved at pH 9.0.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=106697Documentos Relacionados
- Carbon and nitrogen substrate utilization by archival Salmonella typhimurium LT2 cells
- Characterization of a New Operon, as-48EFGH, from the as-48 Gene Cluster Involved in Immunity to Enterocin AS-48
- Isolation and characterization of the Salmonella typhimurium LT2 xylose regulon.
- Structural characterization of the Salmonella typhimurium LT2 umu operon.
- Genetic characterization of Salmonella typhimurium LT2 ara mutations.