Respiratory syncytial virus serology by a simplified enzyme-linked immunosorbent assay.
AUTOR(ES)
Steinhoff, M C
RESUMO
A simplified enzyme-linked immunosorbent assay (ELISA) which utilized commercially available reagents was developed for respiratory syncytial virus (RSV)-specific immunoglobulin G. An analysis of the inherent variation of the assay allowed the setting of strict criteria for determining a significant change in RSV antibodies. The ELISA was more sensitive than the standard complement fixation or microneutralization tests in a carefully studied group of 32 RSV-infected adults. The ELISA correlated closely with complement fixation serological testing in 25 patients. The use of purified antigens might allow the development of a more sensitive ELISA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=273606Documentos Relacionados
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