Resolution and Reconstitution of the Phosphatidate-Synthesizing System of Rat-Liver Microsomes
AUTOR(ES)
Yamashita, Satoshi
RESUMO
The phosphatidate-synthesizing system of rat-liver microsomes was resolved into two component enzymes, glycerolphosphate acyltransferase and 1-acylglycerolphosphate acyltransferase. The resolution is effected by sucrose density gradient centrifugation in the presence of a nonionic detergent, Triton X-100. Combination of both enzymes results in reconstitution of the phosphatidate-synthesizing system. These results establish that two distinct enzymes, glycerolphosphate acyltransferase and 1-acylglycerolphosphate acyltransferase, are required for synthesis of phosphatidic acid from sn-glycerol 3-phosphate.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=389799Documentos Relacionados
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