Resistance of oxidative stress in biofilm and planktonic cells
AUTOR(ES)
Jakubowski, Witold, Walkowiak, Bogdan
FONTE
Braz. arch. biol. technol.
DATA DE PUBLICAÇÃO
2015-04
RESUMO
This work studied the susceptibility of biofilm produced by E. coli to oxidative stress, and compared the components of free radicals defences: level of glutathione, catalase and dismutase activities in planktonic and biofilm located cells. Results showed the diversity of responses to oxidative stress in bacterial cells in log or stationary phases in both planktonic and biofilm forms. The bacteria were exposed to free-radical donors (H2O2, tBOOH, menadione, SIN-1 or peroxynitrite) in a wide range of final concentrations, from 0.5 to 10mM. Different level of toxicity of individual donors, independence of cell type (planktonic forms or biofilm) and phases of growth were observed. The highest oxidative stress resistance was observed for the cells in logarithmic phase of growth treated with H2O2, both in planktonic and biofilm forms, whereas for the cells in stationary phase, the highest resistance was observed for menadione. These results showed higher efficiency of agents based on superoxide anion donors in combating bacteria colonizing abiotic surfaces stainless steel (AISI 316L).
Documentos Relacionados
- Heavy Metal Resistance of Biofilm and Planktonic Pseudomonas aeruginosa
- Multidrug-resistant Candida glabrata strains obtained by induction of anidulafungin resistance in planktonic and biofilm cells
- Farnesol in combination with N-acetylcysteine against Staphylococcus epidermidis planktonic and biofilm cells
- Use of Merocyanine 540 for Photodynamic Inactivation of Staphylococcus aureus Planktonic and Biofilm Cells
- Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosa