Rescue of myeloid lineage-committed preprogenitor cells from cytomegalovirus-infected bone marrow stroma.
AUTOR(ES)
Busch, F W
RESUMO
The effect of murine cytomegalovirus on myelopoiesis was studied in long-term bone marrow culture to find an in vitro correlate for the lethal virus interference with bone marrow reconstitution (W. Mutter, M. J. Reddehase, F. W. Busch, H.-J. Bühring, and U. H. Koszinowski, J. Exp. Med. 167:1645-1658, 1988). The in vitro generation of granulocyte-monocyte progenitors (CFU-GM) discontinued after infection of the stromal cell layer, whereas the proliferation and differentiation of CFU-GM to granulocyte-monocyte colonies remained unaffected. A protocol was established to probe the functional integrity of earlier hematopoietic cells. Pre-CFU-GM (the progenitors of the CFU-GM) could be recovered from an infected bone marrow donor culture by transfer onto an inductive recipient stromal cell layer. Thus, at least in vitro, infection of bone marrow stroma appears to be the only cause of the defect in myelopoiesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=239843Documentos Relacionados
- Enhanced capacity of DNA repair in human cytomegalovirus-infected cells.
- Production of alpha and gamma interferons by spleen cells from cytomegalovirus-infected mice.
- Nonviral Microbodies with Viral Antigenicity Produced in Cytomegalovirus-Infected Cells
- Generation of functional clonal cell lines from human bone marrow stroma.
- Selective cytotoxicity of ricin A chain immunotoxins towards murine cytomegalovirus-infected cells.