Rescue of a non-viable accession and rapd analysis of recovered plants of Arachis retusa.

AUTOR(ES)

GAGLIARDI, R.F.

FONTE

Pesquisa Agropecuária Brasileira

DATA DE PUBLICAÇÃO

2011

RESUMO

In vitro regeneration of Arachis retusa was examined for the purpose of germplasm renewal and conservation. Random amplified polymorphic DNA (RAPD) fingerprinting was used to evaluate the genetic stability of plants derived from embryo axes and apical segments. Ten arbitrary decamer primers were screened and five of them were selected. Ninety genomic regions were evaluated, with an average of 18 loci per clone. All amplified segments were monomorphic. The results indicate that recovered plants are genetically stable at the assessed genomic regions and that both regeneration processes are suitable for in vitro germplasm preservation of Arachis species.

ASSUNTO(S)

germplasm conservation micropropagation molecular characterization genetic stability conservação de germoplasma micropropagação caracterização molecular estabilidade genética

Documentos Relacionados

• Extreme non-viable candidates and quota maneuvering in Brazilian legislative elections
• Decolourization of textile dye effluent by non-viable biomass of Aspergillus fumigatus
• Grazing of particle-associated bacteria-an elimination of the non-viable fraction
• A functional-morphological study of the tidemark region of articular cartilage maintained in a non-viable physiological condition.
• Influence of substrates and in vitro preconditioning treatments on ex vitro acclimatization of Arachis retusa.