Replication of single-stranded DNA templates by primase-polymerase complexes of the yeast, Saccharomyces cerevisiae.
AUTOR(ES)
Biswas, E E
RESUMO
A partially purified primase-polymerase complex from the yeast, Saccharomyces cerevisiae, was capable of replicating a single stranded circular phage DNA into a replicative form with high efficiency. The primase-polymerase complex exhibited primase activity and polymerase activity on singly primed circular ssDNA as well as on gapped DNA. In addition, it was able to replicate an unprimed, single-stranded, circular phage DNA through a coupled primase-polymerase action. On Biogel A-O.5m filtration the primase-polymerase activities appeared in the void volume, demonstrating a mass of greater than 500 kilodaltons. Primase and various primase-polymerase complexes synthesized unique primers on single stranded DNA templates and the size distribution of primers was dependent on the structure of the DNA and the nature of the primase-polymerase assembly.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=338305Documentos Relacionados
- Circular single-stranded RNA replicon in Saccharomyces cerevisiae.
- Homologous recombination between single-stranded DNA and chromosomal genes in Saccharomyces cerevisiae.
- Replication by a single DNA polymerase of a stretched single-stranded DNA
- Production of single-stranded DNA templates by exonuclease digestion following the polymerase chain reaction.
- Fork-like DNA templates support bypass replication of lesions that block DNA synthesis on single-stranded templates
