Removal of 3'-OH-terminal nucleotides from blunt-ended long terminal repeat termini by the avian retrovirus integration protein.

AUTOR(ES)

Vora, A C

RESUMO

The avian myeloblastosis virus integration protein (IN) was capable of removing a specific set of 3'-OH-terminal nucleotides from blunt-ended long terminal repeat (LTR) substrates which resembled linear viral DNA in vivo. The 3'-OH-recessed ends map to the in vivo site of integration on linear viral DNA. The linear DNA plasmid substrate was formed by the generation of a unique DraI restriction enzyme site (TTT/AAA) at the circle junction of a 330-bp tandem LTR-LTR insert. IN preferentially released the three T nucleotides from the minus strand of the U3 LTR substrate compared with its ability to remove the three T nucleotides from the plus strand of the U5 LTR substrate. It was also observed that IN was capable of cleaving a non-LTR DNA substrate containing sequence homology to the U5 LTR terminus.

Documentos Relacionados

• A one tube reaction for the synthesis of blunt-ended double-stranded cDNA.
• Turbo cloning: a fast, efficient method for cloning PCR products and other blunt-ended DNA fragments into plasmids.
• Blunt-ended ligation can be used to produce DNA ladders with rung spacing as large as 0.17 Mb.
• Concerted integration of linear retroviral DNA by the avian sarcoma virus integrase in vitro: dependence on both long terminal repeat termini.
• Spontaneous variation and synthesis in the U3 region of the long terminal repeat of an avian retrovirus.