Relatedness of the double-stranded RNAs present in yeast virus-like particles.
AUTOR(ES)
Bruenn, J
RESUMO
The relatedness of several double-stranded RNAs (dsRNA's) present in the virus-like particles of yeast was examined by T1 fingerprint analysis. The dsRNA's examined were L, the dsRNA encoding the capsid polypeptide of yeast virus-like particles; M, which appears to code for a toxic polypeptide and for resistance to the effects of the toxin; and two S dsRNA's present in particles analogous to the defective interfering particles of animal viruses. S3, a dsRNA of 0.46 X 10(6) daltons, was derived entirely from M, a dsRNA of 1.2 X 10(6) daltons. S1, a dsRNA of 0.92 X 10(6) daltons, was a duplication of S3. This conclusion has also been reached independently by heteroduplex mapping techniques (H. M. Fried and G. R. Fink, personal communication). S1 and S3, at least in one yeast strain, were unstable in sequence, apparently due to the accumulation of sequence variants of the same molecular weight. L was a species of 3 X 10(6) daltons, unrelated in sequence to M, S1, or S3. S1, S3, and M had a 3' T1 dodecanucleotide in common.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=525901Documentos Relacionados
- The double-stranded RNA in Trichomonas vaginalis may originate from virus-like particles.
- Replication of double-stranded RNA of the virus-like particles in Saccharomyces cerevisiae.
- Complex of Virus-Like Particles Containing Double-Stranded RNA from Thielaviopsis basicola
- In vitro L-A double-stranded RNA synthesis in virus-like particles from Saccharomyces cerevisiae.
- Virus-like particle capsid proteins encoded by different L double-stranded RNAs of Saccharomyces cerevisiae: their roles in maintenance of M double-stranded killer plasmids.