Regulatory DNA required for vnd/NK-2 homeobox gene expression pattern in neuroblasts
AUTOR(ES)
Shao, Xiaoping
FONTE
The National Academy of Sciences
RESUMO
Vnd/NK-2 protein was detected in 11 neuroblasts per hemisegment in Drosophila embryos, 9 medial and 2 intermediate neuroblasts. Fragments of DNA from the 5′-flanking region of the vnd/NK-2 gene were inserted upstream of an enhancerless βgalactosidase gene in a P-element and used to generate transgenic fly lines. Antibodies directed against Vnd/NK-2 and β-galactosidase proteins then were used in double-label experiments to correlate the expression of β-galactosidase and Vnd/NK-2 proteins in identified neuroblasts. DNA region A, which corresponds to the −4.0 to −2.8-kb fragment of DNA from the 5′-flanking region of the vnd/NK-2 gene was shown to contain one or more strong enhancers required for expression of the vnd/NK-2 gene in ten neuroblasts. DNA region B (−5.3 to −4.0 kb) contains moderately strong enhancers for vnd/NK-2 gene expression in four neuroblasts. Hypothesized DNA region C, whose location was not identified, contains one or more enhancers that activate vnd/NK-2 gene expression only in one neuroblast. These results show that nucleotide sequences in at least three regions of DNA regulate the expression of the vnd/NK-2 gene, that the vnd/NK-2 gene can be activated in different ways in different neuroblasts, and that the pattern of vnd/NK-2 gene expression in neuroblasts of the ventral nerve cord is the sum of partial patterns.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=117523Documentos Relacionados
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