Regulation of the class II-associated invariant chain gene in normal and mutant B lymphocytes.

AUTOR(ES)

Doyle, C

RESUMO

The invariant chain protein is intracellularly associated with class II major histocompatibility proteins. In many cases, the expression of these molecules appears to be regulated in a similar manner. Contained within the promoter of the invariant chain gene are sequences (X and I gamma 1) that are similar to the X and Y box elements of class II genes, suggesting that these sequences might be involved in its regulation. DNase I footprinting reveals additional cis-acting elements (I gamma 2 and I gamma 3) that contain sequence similarities to NF-kappa B and/or H2TF1/KBF1 recognition sequences. A series of fusion constructs with the chloramphenicol acetyltransferase reporter gene were used to analyze the role of these sequences (I gamma 1, I gamma 2, I gamma 3, and X and Y elements) in both normal and mutant B lymphocytes. These data suggest the likelihood of multiple X box proteins in B cells, which can act as both negative and positive regulatory factors.

Documentos Relacionados

• Direct observation of disordered regions in the major histocompatibility complex class II-associated invariant chain.
• Shared promoter elements between a viral superantigen and the major histocompatibility complex class II-associated invariant chain.
• Proteolysis of the class II-associated invariant chain generates a peptide binding site in intracellular HLA-DR molecules.
• Proteolysis of major histocompatibility complex class II-associated invariant chain is regulated by the alternatively spliced gene product, p41.
• Human cathepsin S, but not cathepsin L, degrades efficiently MHC class II-associated invariant chain in nonprofessional APCs