Regulation of protein kinase C activity in neuronal differentiation induced by the N-ras oncogene in PC-12 cells.
AUTOR(ES)
Lacal, J C
RESUMO
Expression of the N-ras oncogene under the control of the glucocorticoid-responsive promoter in the pheochromocytoma cell line UR61, a subline of PC-12 cells, has been used to investigate the differentiation process to neuronal cells triggered by ras oncogenes (I. Guerrero, A. Pellicer, and D. E. Burstein, Biochem. Biophys. Res. Commun. 150:1185-1192, 1988). Using ras-inducible cell lines, we observed that expression of the oncogenic N-ras p21 protein interferes with the ability of phorbol esters to induce downregulation of protein kinase C. This effect was associated with the appearance of immunologically detectable protein kinase C as well as the activity of the enzyme as analyzed either by binding of [3H]phorbol-12,13-dibutyrate in intact cells or by in vitro kinase activity. These results indicate a relationship between ras p21 and protein kinase C in neuronal differentiation in this model system. Comparison to the murine fibroblast system suggests that this relationship may be functional.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=360662Documentos Relacionados
- Mechanism of activation of an N-ras oncogene of SW-1271 human lung carcinoma cells.
- Oncogene N-ras mediates selective inhibition of c-fos induction by nerve growth factor and basic fibroblast growth factor in a PC12 cell line.
- Mutações do oncogene N-ras em leucemias agudas
- Coordinate N-ras mRNA up-regulation with mutational activation in tumorigenic guinea pig cells.
- The dual-specificity CLK kinase induces neuronal differentiation of PC12 cells.