Regulation of osmC Gene Expression by the Two-Component System rcsB-rcsC in Escherichia coli

AUTOR(ES)

Davalos-Garcia, Marcela

FONTE

American Society for Microbiology

RESUMO

The Escherichia coli osmC gene encodes an envelope protein of unknown function whose expression depends on osmotic pressure and growth phase. The gene is transcribed from two overlapping promoters, osmCp1 and osmCp2. Several factors regulating these promoters have been reported. The leucine-responsive protein Lrp represses osmCp1 and activates osmCp2, the nucleoid-associated protein H-NS represses both promoters, and the stationary-phase sigma factor ςs specifically recognizes osmCp2. This work reports the identification of an additional regulatory element, the two-component system rcsB-rcsC, affecting positively the distal promoter osmCp1. The response regulator of the system, RcsB, does not affect expression of the proximal promoter osmCp2. Deletion analysis located the site necessary for RcsB activation just upstream of osmCp1. In vitro transcription experiments and gel mobility shift assays demonstrated that RcsB stimulates RNA polymerase binding at osmCp1.

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