Regulation of enteropathogenic Escherichia coli (EPEC) adhesion by genes related to nutrional shortage and stress. / Regulação da adesão de Escherichia coli enteropatogênica (EPEC) por genes de resposta à limitação nutricional e estresse.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Enteropathogenic E. coli (EPEC) is one of the causes of diarrhea in children. Phosphate (Pi) shortage induces transcription of the genes known as the PHO regulon. These genes are controlled by the Pst system, that is also a high-affinity Pi transporter, and represses PHO expression under Pi-replete conditions. PHO is also controlled by the two-component system PhoB/PhoR. Deletion of the pst operon reduced the adhesion of EPEC to epithelial cells in vitro due to a decrease in the expression of the regulators PerA and PerC that in turn control the expression of genes related to adhesion. The constitutive expression of the PHO genes in the pst mutant was not the cause of adhesion inhibition. Expression of bfp and the regulators PerA and PerC was also dependent on ppGpp, an alarmone involved in the regulation of genes related to nutrient limitation. On the other hand, RpoS, the factor that controls the general stress response, negatively affected EPEC adhesion and bfpA expression.

ASSUNTO(S)

ppgpp regulação gênica stress operon pst microbial genetics estresse gene regulation escherichia coli (patogenicidade) rpos host-parasite (microbiology) genética microbiana escherichia coli (pathogenicity) genética bacteriana bacterial genetics adhesion adesão pst operon rpos microbiologia ppgpp relações hospedeiro-parasita (microbiologia) microbiology

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