Regulation of cysteine-rich intestinal protein by dexamethasone in the neonatal rat.

AUTOR(ES)

Levenson, C W

RESUMO

The cysteine-rich intestinal protein (CRIP) is an intestinal zinc-binding protein containing a single copy of a cysteine-rich domain known as the LIM motif. CRIP mRNA and protein levels increased in the rat small intestine throughout the suckling period, reaching highest levels by the late weanling stage. A similar developmental pattern of CRIP protein levels was also detected by an increase in zinc binding to CRIP-containing HPLC fractions of intestinal cytosol. Administration of the synthetic glucocorticoid hormone dexamethasone to neonates caused the precocious rise of CRIP mRNA and protein. In adult rats, CRIP mRNA levels were not significantly altered by dexamethasone. Maximal CRIP mRNA content was detected in cells from the mid-villus, as confirmed by expression of cryptdin mRNA. In this report we show the glucocorticoid regulation of the LIM motif-containing protein CRIP and suggest that glucocorticoid hormones play a role in developmental regulation of CRIP.

Documentos Relacionados

• Cysteine-rich intestinal protein binds zinc during transmucosal zinc transport.
• Developmental regulation of a gene that encodes a cysteine-rich intestinal protein and maps near the murine immunoglobulin heavy chain locus.
• A cysteine-rich nuclear protein activates yeast metallothionein gene transcription.
• Isolation and developmental expression of a rat cDNA encoding a cysteine-rich zinc finger protein.
• Importance of the cysteine-rich carboxyl-terminal half of V protein for Sendai virus pathogenesis.