Regeneração de plantas de alho (cv. Chonan), via suspensão celular

AUTOR(ES)
DATA DE PUBLICAÇÃO

1991

RESUMO

Garlic (Allium sativum L.) is a vegetable that is propogated exclusively by vegetative means and it is difficult therefore, to establish a classical breeding programme with genetic recombination through meiotic processes. Hence, in this specie, tissue culture is viewed as a viable alternative to induce and detect variability anel/or mutations. Among the tissue culture techniques available, cell suspension and protoplast culture appear to be good systems for this kind of study. However, for garlic, no adequate methology exists for plant regeneration from cell suspension or protoplast culture. Considering that cell suspension is more versatile than the protoplast technique, the main objective of this research was to establish a cell suspension protocol that inc1udes: production of friable calli (1), establish cells in liquid culture (2), callus induction by plating (3), plant regeneration (4) and bulb formation (5). 1. With regard to friable calli production, these were obtained from aged and compact cv. Chonan callus, using a MS modified medium denominated MSF and whose composition was: salts: MS; vitamins (1.0 mg/l): calcium pantothenate, pyridoxine - HCL, thiamine - HCL and nicotinic acid; organic (mg/1): inositol 100.00, casein hydrolysate 250.00, sucrose non-PA 30.000,00, hormones: 2,4-0 1,0 mg/l. 2. De basal medium was used to establish the cell suspensi°!l cultures. This was a modification of the MS medium, whose composition was: salts: M5, except NH4NO3/3 and FE-EOTA/2; vitamins: as in MSF, but at 3.0 mg/1; organics (mg/l): inositol 100.00, sucrose P.A. 20.000,00, L-glutamine 146.00; hormones: 2,4-0 and BAP 0.5 mg/1 respectively. ... Note: The complete abstract is available with the full electronic digital thesis or dissertations.

ASSUNTO(S)

fisiologia vegetal alho - cultivo

ACESSO AO ARTIGO

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