Real-Time PCR Assay Targets the 23S-5S Spacer for Direct Detection and Differentiation of Legionella spp. and Legionella pneumophila
AUTOR(ES)
Herpers, Bjorn L.
FONTE
American Society for Microbiology
RESUMO
A real-time PCR for the ABI Prism 7000 system targeting the 23S-5S spacer of Legionella spp. was developed. Simultaneous detection and differentiation of Legionella spp. and Legionella pneumophila within 90 min and without post-PCR melting-curve analysis was achieved using two TaqMan probes. In sputum samples from 23 controls and 17 patients with legionellosis, defined by positive culture, urinary antigen testing, or seroconversion, 94% sensitivity and 100% specificity were observed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=254366Documentos Relacionados
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