Rapid Identification of Staphylococcus aureus by Using Fluorescent Staphylocoagulase Assays
AUTOR(ES)
Holliday, M. G.
FONTE
American Society for Microbiology
RESUMO
Two rapid (1-h) assays for the detection of Staphylococcus aureus staphylocoagulase were developed by using the fluorogenic thrombin substrates N-t-boc-Val-Pro-Arg-7-amido-4-methylcoumarin (VPA) and N-t-boc-β-benzyl-Asp-Pro-Arg-7-amido-4-methylocoumarin (BB). The assays were compared to the tube coagulase test and latex agglutination (LA) (Sanofi Diagnostics Pasteur, Guildford, Surrey, United Kingdom) by using 406 clinical isolates of staphylococci, and they produced positive and negative predictive values of 99.2 and 99.1% for LA, 98.9 and 92.7% for VPA, and 98.9 and 99.1% for BB. Fluorescent assays used colonies from solid media, thereby eliminating the need for broth cultures, and were performed in microtiter trays, thus making them suitable for large-scale screening.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=88673Documentos Relacionados
- Specific Identification of Staphylococcus aureus by Staphychrom II, a Rapid Chromogenic Staphylocoagulase Test
- Comparison of rapid identification assays for Staphylococcus aureus.
- Rapid identification of Staphylococcus aureus by using lysostaphin sensitivity.
- Rapid and direct staphylocoagulase assay that uses a chromogenic substrate for identification of Staphylococcus aureus.
- Species-Specific and Ubiquitous-DNA-Based Assays for Rapid Identification of Staphylococcus aureus