Rapid identification of smooth Brucella species with a monoclonal antibody.

AUTOR(ES)

Roop, R M

RESUMO

A colony blot enzyme-linked immunosorbent assay was developed for the rapid identification of smooth Brucella species, i.e., Brucella abortus, B. melitensis, and B. suis. Bacterial colonies from plates were blotted onto nitrocellulose disks, lysed by immersion in chloroform, and reacted with BRU 38, a rat monoclonal antibody with specificity for the O side chain of B. abortus. Reaction with anti-rat immunoglobulin G conjugated to horseradish peroxidase and development in 4-chloro-1-naphthol resulted in colonies of naturally occurring smooth Brucella species staining purple. Results could be obtained within 4 h after colonies were visible on plates and individual colonies could be detected. Yersinia enterocolitica serovar O:9 strains were the only other organisms tested which showed cross-reaction by using this procedure. Because of its speed, sensitivity, and specificity, this technique should be very useful for identifying smooth Brucella strains in diagnostic laboratories.

Documentos Relacionados

• Rapid detection of respiratory syncytial virus with a monoclonal antibody.
• Identification of a species-specific antigen in Legionella pneumophila by a monoclonal antibody.
• Alkaline phosphatase capture test for the rapid identification of Escherichia coli and Shigella species based on a specific monoclonal antibody.
• Identification of Vibrio vulnificus O serovars with antilipopolysaccharide monoclonal antibody.
• Rapid identification of legionellae by a colony blot assay based on a genus-specific monoclonal antibody.