Rapid fluorogenic assay for differentiation of the Candida parapsilosis group from other Candida spp.
AUTOR(ES)
Smitka, C M
RESUMO
A 30-min fluorogenic test was developed for differentiation of members of the Candida parapsilosis group from other Candida species commonly encountered in clinical material. The fluorogenic substrate, 4-methylumbelliferyl-beta-D-glucoside, was utilized to assay beta-glucosidase activity. A total of 50 C. parapsilosis isolates and 135 isolates of four other Candida species were tested. Assay sensitivity and specificity were 100 and 99.3%, respectively. The procedure was adapted for use with a spectrofluorometer.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=267266Documentos Relacionados
- New plate medium for facilitated differentiation of Salmonella spp. from Proteus spp. and other enteric bacteria.
- A 96-well epifluorescence assay for rapid assessment of compounds inhibitory to Candida spp.
- Candida orthopsilosis and Candida metapsilosis spp. nov. To Replace Candida parapsilosis Groups II and III
- Development of a test system for rapid differentiation of Neisseria and Haemophilus spp.
- Fluorogenic assay for rapid detection of Escherichia coli in food.