Rapid detection of enteric adenovirus and rotavirus: a simple method using polyacrylamide gel electrophoresis.
AUTOR(ES)
Moosai, R B
RESUMO
A simple and rapid procedure for identifying adenovirus and rotavirus in stool extracts has been developed. The technique is based on polyacrylamide gel electrophoresis of the virus nucleic acid, but sample preparation is straightforward and does not entail phenol extraction or the use of a radioactive label. Furthermore, processing is not influenced by the amount of specimen obtained and is thus suitable for application as a batch testing method. This approach removes the need for specific antisera, which are not readily available since most of these viruses cannot be grown using routine tissue culture procedures. Trials in this laboratory have indicated that the technique is of comparable sensitivity to electron microscopy.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=499046Documentos Relacionados
- Rapid, simple method of preparing rotaviral double-stranded ribonucleic acid for analysis by polyacrylamide gel electrophoresis.
- A simple method to renature DNA-binding proteins separated by SDS-polyacrylamide gel electrophoresis.
- A simple and rapid method for preparing yeast chromosomes for pulsed field gel electrophoresis.
- A simple method for identification of point mutations using denaturing gradient gel electrophoresis.
- Proceedings: Molecular weight markers for polyacrylamide gel electrophoresis.