Rapid-Cycle PCR for Detection and Typing of Mycoplasma pneumoniae in Clinical Specimens

AUTOR(ES)

Kong, Fanrong

FONTE

American Society for Microbiology

RESUMO

We designed several new primers and modified previously described species- and type-specific primers targeting the Mycoplasma pneumoniae P1 adhesin gene. Optimized thermal profiles allowed one-step or nested PCR to be completed in less than 1 h. In 10 patients with pneumonia, M. pneumoniae type 1 was identified in 3 and type 2 in 7.

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