Radioimmunoassay for Type A Toxin of Clostridium botulinum
AUTOR(ES)
Boroff, Daniel A.
RESUMO
A preparation of pure type A toxin of Clostridium botulinum was labeled with 131I in the presence of chloramine-T and carrier-free isotope. The radioactive toxin (131I Tox) was used in a radioimmunoassay procedure similar to that of Berson and Yalow. Dilutions of antibody to the toxin, capable of binding 50% of the added 131I Tox, were mixed with a sample of the labeled toxin and various concentrations of unlabeled toxin (‚Cold’ Tox). When concentration of Cold Tox were plotted against the ratio 131I bound/131I not bound, a standard curve was established that could be used to estimate the concentration of Cold Tox in a test mixture. This assay was sensitive to as little as 100 mouse minimum lethal dose and was highly specific for the serological type of the toxin used.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=380858Documentos Relacionados
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