Radiation hybrid mapping of the zebrafish genome
AUTOR(ES)
Hukriede, Neil A.
FONTE
The National Academy of Sciences
RESUMO
The zebrafish is an excellent genetic system for the study of vertebrate development and disease. In an effort to provide a rapid and robust tool for zebrafish gene mapping, a panel of radiation hybrids (RH) was produced by fusion of irradiated zebrafish AB9 cells with mouse B78 cells. The overall retention of zebrafish sequences in the 93 RH cell lines that constitute the LN54 panel is 22%. Characterization of the LN54 panel with 849 simple sequence length polymorphism markers, 84 cloned genes and 122 expressed sequence tags allowed the production of an RH map whose total size was 11,501 centiRays. From this value, we estimated the average breakpoint frequency of the LN54 RH panel to correspond to 1 centiRay = 148 kilobase. Placement of a group of 235 unbiased markers on the RH map suggests that the map generated for the LN54 panel, at present, covers 88% of the zebrafish genome. Comparison of marker positions in RH and meiotic maps indicated a 96% concordance. Mapping expressed sequence tags and cloned genes by using the LN54 panel should prove to be a valuable method for the identification of candidate genes for specific mutations in zebrafish.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=22281Documentos Relacionados
- A Radiation Hybrid Map of the Cat Genome: Implications for Comparative Mapping
- Characterization of whole genome radiation hybrid mapping resources for non-mammalian vertebrates.
- The LN54 Radiation Hybrid Map of Zebrafish Expressed Sequences
- Radiation hybrid mapping.
- Panel of Microsatellite Markers for Whole-Genome Scans and Radiation Hybrid Mapping and a Mouse Family Tree