Quantitive Autoradiographic Study of Competence and Deoxyribonucleic Acid Incorporation in Bacillus subtilis
AUTOR(ES)
Somma, Sergio
RESUMO
The relationships among deoxyribonucleic acid (DNA) uptake, transformation, and autoradiographic labeling were investigated. It is shown that: (i) autoradiography is a good method for measuring the total fraction of competent cells able to incorporate transforming DNA; in our best experiment that fraction was 11.5%. (ii) Computation of the fraction of competent cells in a culture of Bacillus subtilis, by comparing the frequencies of single and double transformants for two unlinked markers, gives values which are somewhat different from those obtained by auto-radiography. (iii) On the average, a competent cell of B. subtilis irreversibly takes up from 0.9 × 10−10 to 1.4 × 10−10 μg of DNA; this amount represents about 1/55 to 1/35 of the bacterial chromosome weighing 5 × 10−9 μg, and corresponds to three to six DNA molecules having a molecular weight of 1.6 × 107.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=250401Documentos Relacionados
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