Quantitation of DNA Sequences in Environmental PCR Products by a Multiplexed, Bead-Based Method

AUTOR(ES)

Spiro, Alexander

FONTE

American Society for Microbiology

RESUMO

A first application of a multiplexed, bead-based method is described for determining the abundances of target sequences in an environmental PCR product. Target sequences as little as 0.3% of the total amount of DNA can be quantified. Tests were conducted on 16S ribosomal DNA sequences from microorganisms collected from contaminated groundwater.

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