Quantification of tryptophan in plasma by high performance liquid chromatography
AUTOR(ES)
Pinhati, Renata Romanholi, Polonini, Hudson Caetano, Brandão, Marcos Antônio Fernandes, Raposo, Nádia Rezende Barbosa, Corchs, Felipe, Gattaz, Wagner Farid
FONTE
Química Nova
DATA DE PUBLICAÇÃO
2012
RESUMO
A simple, rapid and selective method using high-performance liquid chromatography with ultraviolet detection (267 nm) was applied for the determination of tryptophan in plasma. Separation was carried out on a C18 column (150 x 4.6 mm internal diameter) in 6 min. The mobile phase consisted of 5 mM the sodium acetate and acetonitrile (92:8, v/v). The method was shown to be precise and accurate, and good recovery of analyte was achieved, characterizing the method as efficient and reliable for use in laboratory analysis.
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