Quality of cellular attachment to various root-end filling materials
AUTOR(ES)
AL-Hiyasat, Ahmad S., Al-Sa'Eed, Oula R., Darmani, Homa
FONTE
Journal of Applied Oral Science
DATA DE PUBLICAÇÃO
2012-02
RESUMO
OBJECTIVES: This study investigated cellular attachment to 6 root-end filling materials as a measure of the biocompatibility of the materials. MATERIAL AND METHODS: Class I retrograde cavities were prepared in root slices and these cavities were filled with the test materials, and incubated with Balb/C 3T3 fibroblasts for 24 h. Root slices with the cavities left empty served as the controls. The root slices were then processed for scanning electron microscopy, and were viewed to assess the quality of cellular attachment by observing the shape of cells, spread, and membrane outline. RESULTS: The best cellular attachment was observed at MTA and Geristore surfaces: cells exhibited characteristic elongated fibroblastic morphology, with projections of lamellipodia, filopodia, blebs, and microvilli from their surfaces, reflecting good attachment to the material. Fibroblasts attached poorly to the surfaces of IRM, Super EBA, KetacFil and Retroplast. Furthermore, the cells did not attach well to the tooth structure next to IRM and Super EBA. CONCLUSIONS: The present study demonstrated a variation in cellular attachment to different root-end filling materials with the best cellular attachment to the surfaces of MTA and Geristore. IRM and Super EBA, Ketac Fil and Retroplast rendered poor attachment.
Documentos Relacionados
- Push-out Bond Strength of Root-end Filling Materials
- New Methodology to Evaluate Bond Strength of Root-End Filling Materials
- Radiopacity evaluation of root-end filling materials by digitization of images
- Solubility and bacterial sealing ability of MTA and root-end filling materials
- Evaluation of physicochemical properties of root-end filling materials using conventional and Micro-CT tests