Purification and Properties of Two RNA Polymerases from Physarum polycephalum
AUTOR(ES)
Burgess, Ann Baker
RESUMO
Two RNA polymerases have been purified from the slime mold Physarum polycephalum, one sensitive and one resistant to α-amanitin. Both enzymes are more active with denatured DNA than native DNA as a template and prefer Mn++ rather than Mg++ as a divalent cation. The α-amanitin-sensitive enzyme shows maximum activity at 0.15 M KCl, whereas the resistant enzyme is most active at very low ionic strength. Analysis of the resistant enzyme on polyacrylamide gels containing sodium dodecyl sulfate shows two subunits present in a 1:1 ratio with molecular weights of 205,000 and 125,000.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=388186Documentos Relacionados
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