Pseudomonas fluorescens UCP 1514 biotechnological potential in degradation of dibenzotiofenol / Potencial biotecnologico de Pseudomonas fluorescens UCP 1514 no no processo degrativo do dibenzotiofeno

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

Crude oil and its distillates contain significant amounts of low-molecular-mass organosulphur compounds such as alkyland cycloalkyl thiols, alkyl- and arylthioethers and aromatic heterocycles based on thiophene. This last group of polycyclic aromatic sulfurated hydrocarbons includes thiophene itself, benzothiophene, dibenzothiophene, and their alkylated derivatives. These compounds have been of concern for decades because they constitute a major class of ubiquitous environmental contaminants found in both air and sea areas. In order to mitigate the consequences of this contamination, such as acid rain and air pollution caused by sulphur dioxide released from the combustion of oils, more and more regulations on sulphur content in petroleum are being established. Biodesulfurization has attracted attention owing to its application to the desulfurization of petroleum due to its mild conditions, lower energy consumption and lower emission of sulphur dioxide. Thus biodesulfurization, which operates under room temperature and pressure conditions, is expected to be a complementary as well as promising alternative to hydrodesulfurization. In this work, we studied the growth kinetics of a bacterium of the strain Pseudomonas fluorescens UCP 1514, which is indigenous in soil, in batch cultures containing dibenzothiophene, a recalcitrant component of fossil fuels and its potential on the desulfurization, in order to use it for reducing the sulfur content of diesel oil in compliance with environmental regulations. The microorganism was capable to metabolize dibenzothiophene and we also identified the physiological mutant colonies that growing on dibenzothiopnhene by DNA amplification with polymerase chain reaction technique and the specific primer BOX.The results showed a decrease of 73% in dibenzothiophene content, indicating that Pseudomonas fluorescens selectively removes sulfur from dibenzothiophene to form biphenyl, indicating that this bacterium shows promising potential for decreasing the sulfur content of fossil fuels, can be used as biological catalyst, on desulfurization and mineralization these compounds, minimizing the problems caused by pollution of this substances

ASSUNTO(S)

environment biotechnology quimica ambiental biodegradação bioremediação degradation biotecnologia bioremediation

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