Proteomic analysis of whey from bovine colostrum and mature milk

AUTOR(ES)

Golinelli, Luciana Pacheco, Conte-Junior, Carlos Adam, Paschoalin, Vânia Margaret Flosi, Silva, Joab Trajano

FONTE

Brazilian Archives of Biology and Technology

DATA DE PUBLICAÇÃO

2011-08

RESUMO

The aim of this study was to standardize a methodology to obtain two-dimensional (2D) maps of whey proteins from bovine colostrum and mature milk, using two-dimensional electrophoresis, in order to identify the minor proteins by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI TOF/TOF MS). A total of 38 proteins were identified, 20 spots in the colostrum whey and 18 in the mature milk whey; 5 of them were identified for the first time.

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