Protein decorated Nanoparticules : Small Unilamellar Lipid Vesicules and Block Copolymers Micelles. / Nanoparticules Décorées par des Protéines : Vésicules Lipidiques Unilamellaires et Micelies à Base de Copolymères à Blocs

Vanessa Schmidt Giacomelli

Protein decorated Nanoparticules : Small Unilamellar Lipid Vesicules and Block Copolymers Micelles. / Nanoparticules Décorées par des Protéines : Vésicules Lipidiques Unilamellaires et Micelies à Base de Copolymères à Blocs

AUTOR(ES)

Vanessa Schmidt Giacomelli

FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

03/07/2008

RESUMO

The formation of protein Annexin-A5 decorated (bio-functionalized) nanoparticles is of particular interest in micelle-mediated target drug delivery, in vivo magnetic resonance imaging, or controlled fabrication of biochips. This work describes an easy access to the synthesis and manipulation of block copolymer nano-objects exhibiting binding ability for the Annexin-A5 protein. Well-defined spherical micelles containing negatively charged phosphonic diacid groups - which are potential binding sites for Annexin-A5 proteins - at their hydrophilic periphery originate from the self-assembly of polystyrene-b-poly(2-phosphatethyl methacrylate-stat-2-hydroxyethyl methacrylate) (PS-b-P(PEMA-stat-HEMA)) and poly(n-butyl acrylate)-b-poly((1-ethoxycarbonyl)vinylphosphonic diacid) (PBuA-b-PECVPD) amphiphilic macromolecules in aqueous media. The above cited block copolymers were synthesized mainly via Atom Transfer Radical Polymerization (ATRP). The herein discussed approach allows precise control over micellar dimensions and properties such as core radius [i.e., payload capacity), corona width, and density of phosphate groups at the micelle periphery. The polyelectrolyte behavior of block copolymer micelles originated from the self-assembly of block copolymers chains and their binding to Annexin-A5 protein was studied in detail by a combination of potentiometry, static and dynamic light scattering (SLS and DLS), small angle x-ray scattering (SAXS), transmission electron microscopy (TEM) and cryo-transmission electron microscopy (cryo-TEM), quartz crystal microbalance with dissipation monitoring (QCM-D), and polyacrylamide gel electrophoresis (PAGE).

ASSUNTO(S)

copolymères à blocs nano-partículas diffusion de rayon-x light scattering quimica adsorção de proteínas síntese de polímeros micelas de copolímeros em bloco microscopia de transmissão eletrônica block copolymers self-assembly micelles polyelectrolyte behavior x-ray scattering transmission electron microscopy protein decorated nanoparticles annexin-a5 protein atom transfer radical polymerization (atrp) auto-assemblage effet polyélectrolyte diffusion de lumière microscopie électronique à transmission nano-objets décorés par des protéines protéine annexine-a5 espalhamento de luz estático e dinâmico polymérisation radicalaire par transfert d\ atome (atrp).