Prophylactic application of laser light restores L-FABP expression in the livers of rats submitted to partial ischemia
AUTOR(ES)
Vilalva, Kelvin Henrique, Figueira, Rebeca Lopes, Silveira, Marina, Graf, Catarina, Gonçalves, Frances Lanhellas, Sbragia, Lourenço, Gomes, Maria Cecília, Mumic, Fabrícia, Vollet-Filho, José Dirceu, Bagnato, Vanderlei Salvador, D’Albuquerque, Luiz Augusto Carneiro, Castro-e-Silva, Orlando
FONTE
Clinics
DATA DE PUBLICAÇÃO
02/07/2018
RESUMO
OBJECTIVES: The objective of the present study was to evaluate the protective effect of pre-conditioning treatment with laser light on hepatic injury in rats submitted to partial ischemia using mitochondrial function and liver fatty acid binding protein as markers. METHODS: Rats were divided into four groups (n=5): 1) Control, 2) Control + Laser, 3) Partial Ischemia and 4) Partial Ischemia + Laser. Ischemia was induced by clamping the hepatic pedicle of the left and middle lobes of the liver for 60 minutes. Laser light at 660 nm was applied to the liver immediately prior to the induction of ischemia at 22.5 J/cm2, with 30 seconds of illumination at five individual points. The animals were sacrificed after 30 minutes of reperfusion. Blood and liver tissues were collected for analysis of mitochondrial function, determination of malondialdehyde and analysis of fatty acid binding protein expression by Western blot. RESULTS: Mitochondrial function decreased in the Partial Ischemia group, especially during adenosine diphosphate-activated respiration (state 3), and the expression of fatty acid binding protein was also reduced. The application of laser light prevented bioenergetic changes and restored the expression of fatty acid binding protein. CONCLUSION: Prophylactic application of laser light to the livers of rats submitted to partial ischemia was found to have a protective effect in the liver, with normalization of both mitochondrial function and fatty acid binding protein tissue expression.
Documentos Relacionados
- L-FABP and I-FABP expression in newborn rats changes inversely in the model of necrotizing enterocolitis
- L-FABP directly interacts with PPARα in cultured primary hepatocytes
- Gene Expression Patterns of Geese Expression Patterns of L-FABP, Spot 14, OB and APO A1 Genes in Different Tissues of Overfed and Control Geese
- Brain caspase-3 and intestinal FABP responses in preterm and term rats submitted to birth asphyxia
- Ischemic preconditioning and the gene expression of enteric endothelial cell biology of rats submitted to intestinal ischemia and reperfusion