PROPERTIES OF HALVES OF IMMUNOGLOBULIN LIGHT CHAINS*

AUTOR(ES)

Karlsson, F. Anders

RESUMO

Light polypeptide chains from both normal human γG immunoglobulin and Bence-Jones proteins can be cleaved into halves by limited proteolysis with trypsin, pepsin, or papain. The fragments were obtained in yields of up to 22 per cent, had molecular weights of 10,000 to 11,000, and were shown by amino acid analysis and antigenic analysis to correspond to variable or constant regions of light chains. Starch gel electrophoresis in urea suggested that each half consisted of a single polypeptide chain. A fragment from the urine of a myeloma patient corresponded almost exactly to the constant half of a γ-chain and had a compact shape (Stokes radius of 16 Å; frictional ratio of 1.1). Similar Stokes radii were estimated both on fragments from normal urine and fragments produced by proteolysis of normal light chains.

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