Properties of an Immobilized Pesticide-Hydrolyzing Enzyme
AUTOR(ES)
Munnecke, Douglas M.
RESUMO
A bacterial enzyme(s) capable of hydrolyzing nine organophosphate insecticides was covalently bound to glass. The efficiency of this binding reaction ranged from 4 to 17%. Under continuous column operation, the immobilized enzyme(s) had an extrapolated half-life of 280 days. The specific activity of this glass-covalently bound hydrolase activity for parathion varied from 0.035 to 0.15 μmol/min per g of glass. The bound activity increased with decreasing glass particle size; however, the flow resistance also increased. Immobilized enzyme(s) kinetics were approximately 50% slower than those of the free enzyme(s), but there was no significant difference in the effect pH and temperature had on the activity of immobilized and free enzyme(s).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=170716Documentos Relacionados
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