Promoter-proximal tethering elements regulate enhancer-promoter specificity in the Drosophila Antennapedia complex
AUTOR(ES)
Calhoun, Vincent C.
FONTE
The National Academy of Sciences
RESUMO
Insulator DNAs and promoter competition regulate enhancer–promoter interactions within complex genetic loci. Here we provide evidence for a third mechanism: promoter-proximal tethering elements. The Scr-ftz region of the Antennapedia gene complex includes two known enhancers, AE1 and T1. AE1 selectively interacts with the ftz promoter to maintain pair-rule stripes of ftz expression during gastrulation and germ-band elongation. The T1 enhancer, located 3′ of the ftz gene and ≈25 kb 5′ of the Scr promoter, selectively activates Scr expression in the prothorax and posterior head segments. A variety of P element minigenes were examined in transgenic embryos to determine the basis for specific AE1-ftz and T1-Scr interactions. A 450-bp DNA fragment located ≈100 bp 5′ of the Scr transcription start site is essential for T1-Scr interactions and can mediate long-range activation of a ftz/lacZ reporter gene when placed 5′ of the ftz promoter. We suggest that the Scr450 fragment contains tethering elements that selectively recruit T1 to the Scr promoter. Tethering elements might regulate enhancer–promoter interactions at other complex genetic loci.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=123125Documentos Relacionados
- Molecular Analysis of the Zeste-White Interaction Reveals a Promoter-Proximal Element Essential for Distant Enhancer-Promoter Communication
- Long-range enhancer–promoter interactions in the Scr-Antp interval of the Drosophila Antennapedia complex
- Enhancer-promoter communication at the Drosophila engrailed locus
- Enhancer–promoter specificity mediated by DPE or TATA core promoter motifs
- Promoter-Proximal Pausing on the hsp70 Promoter in Drosophila melanogaster Depends on the Upstream Regulator
