Projeto e construção de um eletropermeabilizador de células biológicas / Design and construction of a biological cell electroporator
AUTOR(ES)
Carlos Toshiyuki Matsumi
DATA DE PUBLICAÇÃO
2009
RESUMO
Electropermeabilization is the process of transient increase in the permeability of biological membranes of cells subjected to intense electric fields. This technique is currently still in development and has important clinical and technological applications such as electrochemotherapy and gene transfer. Electroporator is the equipment used in the generation and application of such intense fields. This dissertation presents the design, construction and testing of an electroporator for use in biomedical research. The proposed equipment presents versatility and performance appropriate to allow for different types of experiments with biological tissues or cell suspensions. It consists of a voltage generator with programmable wave form, a high voltage amplifier with high output current capability and a system for transduction of voltage and current in the sample. Both the generation of signal as the measured values in the load are monitored by a program built in LabVIEW environment that triggers a data acquisition card with 16 bits of resolution. The performance of the electronic system developed fully meets the requirements of project. The amplifier can deliver up to 500V and 5A to the load during a time interval enough for the testing of electropermeabilization. The bandwidth, slightly higher than 100kHz and the step response time of the order of 1μs are suitable for performing experiments with different waveforms and different sizes of cells. An important application of the equipment built is demonstrated with an experiment of electropermeabilization in suspension of red cells of rats. This result demonstrated the occurrence of increased conductivity of the sample during stimulation with high-intensity electric field.
ASSUNTO(S)
eletropermeabilização condutividade de suspensão de células conductivity of cell suspensions amplificador de alta tensão high voltage amplifier eletropermeabilizador electroporator automacao eletronica de processos eletricos e industriais electropermeabilization
