Production of Trichoderma reesei cellulases on glucose-containing media.
AUTOR(ES)
Nakari-Setälä, T
RESUMO
The filamentous fungus Trichoderma reesei was shown to secrete active cellobiohydrolase I and the endoglucanase I catalytic core domain into the culture medium when the fungus was grown on glucose-containing medium. The expression of the proteins was driven by the promoters of the elongation factor 1 alpha, tef1, and the unidentified gene for cDNA1. The cDNA1 promoter gave the best yields. The highest amounts of cellobiohydrolase I and the endoglucanase I core, being 50 to 100 mg/liter, accounted for more than half of the total protein secreted by the fungus. The levels obtained with the tef1 promoter were 20 to 50 times lower.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=167665Documentos Relacionados
- Isolation of glucose-containing high-mannose glycoprotein core oligosaccharides.
- BACTERIAL METABOLISM THE INFLUENCE OF PHOSPHATE BUFFER IN CARBOHYDRATE-FREE AND IN GLUCOSE-CONTAINING MEDIA
- Glucose-induced secretion of Trichoderma reesei xylanases.
- Isolation and Characterization of a Catabolite Repression-Insensitive Mutant of a Methanol Yeast, Candida boidinii A5, Producing Alcohol Oxidase in Glucose-Containing Medium
- Improved production of Trichoderma harzianum endochitinase by expression in Trichoderma reesei.