Production of Respirable Vesicles Containing Live Legionella pneumophila Cells by Two Acanthamoeba spp.

AUTOR(ES)

Berk, Sharon G.

FONTE

American Society for Microbiology

RESUMO

Two Acanthamoeba species, fed at three temperatures, expelled vesicles containing living Legionella pneumophila cells. Vesicles ranged from 2.1 to 6.4 μm in diameter and theoretically could contain several hundred bacteria. Viable L. pneumophila cells were observed within vesicles which had been exposed to two cooling tower biocides for 24 h. Clusters of bacteria in vesicles were not dispersed by freeze-thawing and sonication. Such vesicles may be agents for the transmission of legionellosis associated with cooling towers, and the risk may be underestimated by plate count methods.

Documentos Relacionados

• Apoptosis of Primary-Culture Rat Microglial Cells Induced by Pathogenic Acanthamoeba spp.
• Multiplication of Legionella spp. in tap water containing Hartmannella vermiformis.
• Intracellular growth of Legionella pneumophila within Acanthamoeba castellanii Neff.
• Growth of Legionella pneumophila in Acanthamoeba castellanii enhances invasion.
• Resuscitation of viable but nonculturable Legionella pneumophila Philadelphia JR32 by Acanthamoeba castellanii.