Production of L-serine by Sarcina albida.
AUTOR(ES)
Ema, M
RESUMO
Conditions for the production of microbial L-serine hydroxymethyltransferase and for the conversion of glycine to L-serine were studied. A number of microorganisms were screened for their abilities to form and accululate L-serine from glycine, and Sarcina albida was selected as the best organism. Enzyme activity in this organism as high as 0.12 U/ml could be produced in shaken cultures at 30 degrees C in a medium containing glucose, ammonium sulfate, glycine, yeast extract, and inorganic salts. L-Serine was produced most efficiently by shaking cells at 30 degrees C in a reaction mixture containing 20% glycine, 5 X 10(-3) M formaldehyde, and 3 X 10(-4) M pyridoxal phosphate in yields of 22 mg of broth in 5 days. L-Serine was easily isolated in 84% yields by ion-exchange resin.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=243352Documentos Relacionados
- l-Serine Production by a Mutant of Sarcina albida Defective in l-Serine Degradation
- l-Serine Deaminase of Escherichia coli
- l-Serine Catabolism via an Oxygen-Labile l-Serine Dehydratase Is Essential for Colonization of the Avian Gut by Campylobacter jejuni
- Cometabolism of a Nongrowth Substrate: l-Serine Utilization by Corynebacterium glutamicum
- l-SERINE SPECIFIC DEHYDRASE FROM CLOSTRIDIUM ACIDI-URICI1
