Production of an extracellular milk-clotting activity during development in Myxococcus xanthus.

AUTOR(ES)

Petit, F

RESUMO

We describe here an extracellular proteolytic activity secreted during both growth and submerged development by Myxococcus xanthus DK1622. This activity yields the clotting of kappa-casein at pH 6 and is inhibited by specific inhibitors of aspartic proteases. Secretion of this milk-clotting proteolytic activity (of Mcp) is time regulated during the developmental cycle, with a large increase near 9 h poststarvation, but its production does not require cell-cell contact. The lack of secretion of this activity by several developmental mutants in submerged development conditions shows that Mcp production is developmentally regulated.

Documentos Relacionados

• Cloning and Expression of clt Genes Encoding Milk-Clotting Proteases from Myxococcus xanthus 422
• Milk-Clotting Enzymes From Microorganisms
• Potential of quixaba (Sideroxylon obtusifolium) latex as a milk-clotting agent
• Methylation of macromolecules during development in Myxococcus xanthus.
• Jacaratia corumbensis O. Kuntze a new vegetable source for milk-clotting enzymes