Production and Property of Beta-Lactamases in Streptomyces
AUTOR(ES)
Ogawara, Hiroshi
RESUMO
The production of β-lactamases by 100 strains of Streptomyces was studied. About one-half of the strains produced more than 2.3 U of β-lactamase per ml, and another half produced less than 1.4 U/ml. The amounts of β-lactamases produced by two strains were in the order of those produced by Bacillus cereus 569/H and Bacillus licheniformis 749/C. These Streptomyces enzymes function primarily as penicillinases rather than cephalosporinases. Properties such as pH optimum, substrate specificity, and heat stability suggest that these Streptomyces β-lactamases are closely related to each other. In contrast to bacterial β-lactamases, Streptomyces β-lactamases were insusceptible to inactivation by N-bromosuccinimide and only slightly susceptible to iodine. This suggests that the construction mode of the active site would be different from other β-lactamases. Studies on the minimum inhibitory concentrations of benzylpenicillin to seven Streptomyces strains and on their maximum β-lactamase production indicate that the susceptibility of Streptomyces to penicillin is not directly related to the β-lactamase production.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=429356Documentos Relacionados
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