Produção e caracterização estrutural do exopolissacarídeos secretado pelo ascomiceto Botryosphaeria SP

AUTOR(ES)

Rosangela Maria Steluti

DATA DE PUBLICAÇÃO

2004

RESUMO

The influence of glucose concentration and other carbohydrates (monosaccharides: fructose, galactose, mannose; polyols: mannitol and sorbitol; disaccharides: lactose, sucrose and commercial sucrose; and industrial sugarcane molaless) were compared as sole carbon sources for the production of Botryosphaeran, an exopolysaccharide (EPS) produced by Botryosphaeria sp. The optimum glucose concentration for EPS production was 50g L-1. With the exception of mannitol, the fungus produced EPS on all carbon sources studied, with highest yields occurring with sucrose followed by glucose. All EPS showed exclusively glucose after acid hydrolysis and monosaccharides analysis. FTIR spectroscopy demonstrated the presence of ?-anomers indicating that all the EPS produced by Botryosphaeria sp. on the different carbon sources were essentially b-D-glucan type. The Botryosphaeran produced on glucose as sole carbon source, was isolated from the extracellular fluid by precipitation with ethanol, and purified by gel permeation chromatography to yield a carbohydrate-rich fraction (96%) composed mainly of glucose (98%). Infra-red and 13C-NMR spectroscopy showed that all the glucosidic linkages were in the b-configuration. Data from methylation analysis and Smith degradation indicated that the EPS is a b-(1?3)-D-glucan with approximately 22% side branching on C-6. The products obtained from partial acid hydrolysis demonstrated that the side branches consist of single (1?6)-b- linked glucosyl, and (1?6)-b- linked gentiobiosyl residues.

ASSUNTO(S)

biotecnologia glucanas polissacarídeos ascomicetos fungos - biotecnologia microbiologia industrial biotechnology glucans polysaccharides ascomycetes fungi - biotechnology industrial microbiology

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