Processing and microfiltration of mosquitoes for malaria antigen detection in a rapid dot immunobinding assay.

AUTOR(ES)

Oprandy, J J

RESUMO

Data on a technique for the detection of antigen from arthropod vectors in a dot immunobinding assay are presented. In this system, antigen present in the vector was first solubilized in sodium dodecyl sulfate. The homogenate from this process was microfiltered through a two-membrane sandwich; target antigen molecules passed through the first membrane and were immobilized on the second one. The first membrane was nonbinding and served to impinge debris. The second membrane was a high-protein-binding-capacity hydrophobic polyvinylidene difluoride membrane. High signal-to-noise ratios were produced by this method, which is readily adaptable for field use. This assay was used for malaria sporozoites, but it can serve as a general technique that is applicable to other arthropod vectors and etiologic agents.

Documentos Relacionados

• Detection of antibody to immunodeficiency viruses by dot immunobinding assay.
• Serodiagnosis of rabies by dot immunobinding assay.
• Diagnosis of invasive candidiasis by a dot immunobinding assay for Candida antigen detection.
• Rapid dot-immunobinding assay on nitrocellulose for viral antibodies.
• Specific Dot-Immunobinding Assay for Detection and Enumeration of Thiobacillus ferrooxidans