Problems in detection of cytomegalovirus in urine samples by dot blot hybridization.
AUTOR(ES)
Augustin, S
RESUMO
A hybridization assay for the detection of cytomegalovirus (CMV) in urine specimens was established. Two different DNA fragments were used as hybridization probes: the HindIII L fragment (11.7 kilobases) and the EcoRI J fragment (10.6 kilobases) of the human CMV strain AD169. These probes were used in an isolated and highly purified form and therefore did not cross hybridize with vector sequences. As shown by hybridization with DNA from CMV-infected and uninfected cells, the assay was highly CMV specific and sensitive (detection limit, 750 to 500 fg of CMV DNA). A total of 122 urine specimens were examined by DNA hybridization, virus isolation, and the detection of CMV-induced early nuclear protein. The results coincided in 91% of the samples. The application of DNA hybridization to urine samples, however, is not without problems, and some of the pitfalls and drawbacks are discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=269379Documentos Relacionados
- Rapid detection of cytomegalovirus DNA in urine samples with a dot blot hybridization immunoenzymatic assay.
- Direct detection of molluscum contagiosum virus in clinical specimens by dot blot hybridization.
- Cytomegalovirus in urine: detection of viral DNA by sandwich hybridization.
- Specific detection and typing of adenovirus types 40 and 41 in stool specimens by dot-blot hybridization.
- Dot immunoperoxidase assay using monoclonal antibody for direct detection of cytomegalovirus in urine samples.