Pro-embryogenic cell viability and cell suspension of Byrsonima intermedia A. Juss. / Viabilidade de CÃlulas PrÃ- EmbriogÃnicas e SuspesÃo Celular de Murici-Pequeno

AUTOR(ES)

Luciano Coutinho Silva

DATA DE PUBLICAÇÃO

2009

RESUMO

The Murici-pequeno (Byrsonima intermedia A. Juss) is a fruitful medicinal species of Cerrado which presents difficulties to be propagated by conventional methods. This fact makes necessary the development of alternative methodologies of propagation for this species. In this work it was studied the in vitro seedlings growth according to residual effects of seeds disinfestation with NaOCl solution, using different pH and periods of exposition. It was produced callus from in vitro seedlings leaf segments cultivated in MS medium with 2.4-D (4.5 ÂM). These were subcultivated for three times, with 60 days each. For each subcultivation it was assayed the embryogenic potential using the dyes Aceto-carmine (A.C.) and Evans-blue (E.B.). In addition, FDA and CTT dyes were also used to analyze the callus viability during the subcultivation periods. Finally, a cell suspension was established and its growth analyzed by the cells counting using a Neubauer chamber. Seedlings growth was affected by the pH variation and exposition periods to NaOCl. The cytochemical tests by double staining with A.C. and E.B. allowed the characterization of the embryogenic potential for the three subcultivations by analyzing the areas stained with these dyes. Using CTT, higher callus viability was observed at the 25o day of cultivation. Using the FDA test at the 25Â, 29Â and 21Â days of cultivation, higher number of viable cells for the first, second and third subcultivation, respectively, were observed. The cell suspension analysis showed that use of NAA promotes a higher number of viable cells in the third subcultivation.

ASSUNTO(S)

pro-embryogenic masses fda ttc-tetrazolium fda ctt-tetrazÃlium viabilidade celular fisiologia vegetal byrsomina intermedia a juss byrsonima intermedia a juss massas prÃ-embriogÃnicas cell viability

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