Principles of Site-Specific Recombinase (SSR) Technology
AUTOR(ES)
Bucholtz, Frank
FONTE
MyJove Corporation
RESUMO
Site-specific recombinase (SSR) technology allows the manipulation of gene structure to explore gene function and has become an integral tool of molecular biology. Site-specific recombinases are proteins that bind to distinct DNA target sequences. The Cre/lox system was first described in bacteriophages during the 1980's. Cre recombinase is a Type I topoisomerase that catalyzes site-specific recombination of DNA between two loxP (locus of X-over P1) sites. The Cre/lox system does not require any cofactors. LoxP sequences contain distinct binding sites for Cre recombinases that surround a directional core sequence where recombination and rearrangement takes place. When cells contain loxP sites and express the Cre recombinase, a recombination event occurs. Double-stranded DNA is cut at both loxP sites by the Cre recombinase, rearranged, and ligated ("scissors and glue"). Products of the recombination event depend on the relative orientation of the asymmetric sequences.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2583015Documentos Relacionados
- The catalytic domain of λ site-specific recombinase
- Cre recombinase-mediated site-specific recombination between plant chromosomes.
- Domain of a yeast site-specific recombinase (Flp) that recognizes its target site.
- Inhibition of recombinant human immunodeficiency virus type 1 replication by a site-specific recombinase.
- Site-specific integration of Agrobacterium T-DNA in Arabidopsis thaliana mediated by Cre recombinase.