Prevalence of HHV-8 in blood donors and recipients in the city of São Paulo, by indirect imunofluorescent assay and Nested PCR / "Prevalência do HHV - 8 em doadores de sangue e politransfundidos na cidade de São Paulo, pelas técnicas de imunofluorescência indireta e Nested PCR"

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

The prevalence of antibodies and DNA viral of HHV-8 in 400 donors of blood and 50 receiving of the Fundação Pró-Sangue Hemocentro of São Paulo, through Nested PCR S techniques and IFI for lithic and latent antigens. A seroprevalence of 4% was detected in the donors of blood and 14% in receiving, significantly high in comparison with the prevalence in donors of blood (p = 0,002). There was a significant difference in the prevalence among men 1,8% and 7% in the women (p= 0,012; 95% CI 1,14 - 16,6). They were detected DNA of HHV-8 in the plasma and a donor s 6,3% PBMC, and it was sequenced and submitted to the Gene Bank where presented 100% of identity. This allows to define the transmission tax and the need of introducing methods for this agent s selection

ASSUNTO(S)

herpesvirus humano 8 blood transfusion blood donors transfusÃo indireta de fluorescÊncia para anticorpo polymerase chain reaction herpesvirus 8 human cross-sectional studies fluorescent antibody technique indirect doadores de sangue reaÇÃo em cadeia da polimerase

ACESSO AO ARTIGO

Documentos Relacionados