Prevalence of heat-stable II enterotoxigenic Escherichia coli in pigs, water, and people at farms in Thailand as determined by DNA hybridization.

AUTOR(ES)

Echeverria, P

RESUMO

The DNA hybridization assay employing a 460-base-pair fragment of DNA encoding for the methanol-insoluble form of heat-stable toxin (ST-II) was used to determine the prevalence of ST-II enterotoxigenic Escherichia coli (ETEC) in pigs, people, and water at 57 farms in Sri Racha, Thailand. ST-II ETEC was found in 62 (3%) of 2,110 suckling, 181 (32%) of 560 weaned, and 4 (1%) of 457 adult pigs examined. Of 62 suckling pigs with ST-II ETEC infections 21% had diarrhea, but none of 185 infected older pigs had diarrhea. ST-II ETEC was found more frequently in suckling pigs with diarrhea than without diarrhea (13 of 146 versus 49 of 1,964) (P less than 0.001). ST-II ETEC was detected in water collected from 3 of 57 clay jars containing water used to bathe at three pig farms, in 1 jar used to bathe immediately after working in the barn, and from one farmer who did not have a recent history of diarrhea. Evidence of this organism was not found in 245 other individuals living on the pig farms or in 220 inhabitants and 114 water specimens collected at tapioca farms nearby. In Thailand ST-II ETEC was found in suckling pigs with diarrhea but was infrequently found in humans.

Documentos Relacionados

• Synthetic oligodeoxyribonucleotide probes for detecting heat-stable enterotoxin-producing Escherichia coli by DNA colony hybridization.
• Detection of heat-stable enterotoxigenic Escherichia coli by hybridization with an RNA transcript probe.
• Characterization of the gene encoding heat-stable toxin II and preliminary molecular epidemiological studies of enterotoxigenic Escherichia coli heat-stable toxin II producers.
• Biological evaluation of a methanol-soluble, heat-stable Escherichia coli enterotoxin in infant mice, pigs, rabbits, and calves.
• Repression of heat-stable enterotoxin synthesis in enterotoxigenic Escherichia coli.